As the complexity of the genome increases dramatically from yeast to human, additional proteins have been identified in higher eukaryotes that dictate replication initiation, progression and licensing. Many of these proteins, in addition to their bona fide roles in DNA replication are also required for other cell cycle events including heterochromatin organization, chromosome segregation and centrosome biology. Several components of the pre-RC and pre-IC are highly conserved across all examined eukaryotic species. These events are achieved by the formation of distinct protein complexes that form in a cell cycle-dependent manner. N2 - Faithful duplication of the genome in eukaryotes requires ordered assembly of a multi-protein complex called the pre-replicative complex (pre-RC) prior to S phase transition to the pre-initiation complex (pre-IC) at the beginning of DNA replication coordinated progression of the replisome during S phase and well-controlled regulation of replication licensing to prevent re-replication. T1 - Emerging Players in the Initiation of Eukaryotic DNA Replication In this review, we discuss the newly discovered components and their roles in cell cycle progression.",
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In this review, we discuss the newly discovered components and their roles in cell cycle progression.Ībstract = " Faithful duplication of the genome in eukaryotes requires ordered assembly of a multi-protein complex called the pre-replicative complex (pre-RC) prior to S phase transition to the pre-initiation complex (pre-IC) at the beginning of DNA replication coordinated progression of the replisome during S phase and well-controlled regulation of replication licensing to prevent re-replication.
![supriya g. prasanth lab supriya g. prasanth lab](https://www.embopress.org/cms/asset/3b3d01c7-ed2a-410a-bc82-e77ba5aa8047/embj7600255-fig-0005-m.jpg)
This mechanism of action highlights an important paradigm for the role of a nuclear-retained stable RNA transcript in regulating gene expression.Faithful duplication of the genome in eukaryotes requires ordered assembly of a multi-protein complex called the pre-replicative complex (pre-RC) prior to S phase transition to the pre-initiation complex (pre-IC) at the beginning of DNA replication coordinated progression of the replisome during S phase and well-controlled regulation of replication licensing to prevent re-replication.
![supriya g. prasanth lab supriya g. prasanth lab](https://mcb.illinois.edu/system/news_items/image_mcbs/000/000/491/h304/prasanth2fix_16-9.jpg)
Our findings reveal a role of the cell nucleus in harboring RNA molecules that are not immediately needed to produce proteins but whose cytoplasmic presence is rapidly required upon physiologic stress. Under stress, CTN-RNA is posttranscriptionally cleaved to produce protein-coding mCAT2 mRNA.
![supriya g. prasanth lab supriya g. prasanth lab](https://media.springernature.com/lw685/springer-static/image/art%3A10.1038%2Fncomms4445/MediaObjects/41467_2014_Article_BFncomms4445_Fig2_HTML.jpg)
Interestingly, knockdown of CTN-RNA also downregulates mCAT2 mRNA. The 3�UTR of CTN-RNA contains elements for adenosine-to-inosine editing, involved in its nuclear retention. CTN-RNA is diffusely distributed in nuclei and is also localized to paraspeckles. CTN-RNA is transcribed from the protein-coding mouse cationic amino acid transporter 2 (mCAT2) gene through alternative promoter and poly(A) site usage. We have identified CTN-RNA, a mouse tissue-specific w8 kb nuclear-retained poly(A) + RNA that regulates the level of its protein-coding partner. Multiple mechanisms have evolved to regulate the eukaryotic genome.